Description_x000D_ General description_x000D_ Murashige and Skoog medium is a widely used plant tissue culture growth medium. M&S Basal Medium contains macronutrients that include high levels of nitrate and organic additives such as agar, sugars, vitamins and growth regulators. Important growth regulators frequently added to M&S include IAA (auxin/morphogen) and the Kinetin (cytokinin/cell division promoter)._x000D_ Application_x000D_ Murashige and Skoog medium has been used in plant tissue culture medium for plant transformation study._x000D_ Formula variant_x000D_ With the macro- and micronutrients, and vitamins as described by Murashige and Skoog (1962)._x000D_ Media Formulation_x000D_ Quantity_x000D_ Formulated to contain 4.4 grams of powder per liter of medium._x000D_ Preparation Note_x000D_ Murashige and Skoog medium can be reconstituted from powder or by combining products that are major components of complete M&S medium, such as macronutrient mixtures and vitamin mixtures. Murashige and Skoog medium (M5519) contains the micronutrients and vitamins of the original classic formulation. It can be supplemented with sucrose, agar, auxins (IAA) and cytokinins (Kinetin) to generate a complete medium for growth plant tissue culture.