Description Specificity Recognizes a full-length m2 protein. It has exhibited no cross reactivity with other muscarinic proteins tested so far. SPECIES REACTIVITIES: In Chimpanzee and gorilla the immunogen sequence is 100% identical. Orangutan (130/132), pig (122/132), and dog (122/132). Other species have not been tested. Immunogen GST fusion protein (Rybin, 2000) and part of i3 intercellular loop of human m2 muscarinic acetylcholine receptor (amino acids 225-356) (Accession P08172). Application Anti-Muscarinic Acetylcholine Receptor m2 Antibody is an antibody against Muscarinic Acetylcholine Receptor m2 for use in IH, IP & WB. Immunohistochemistry on rat brain sections. Western blot: 1:200 using ECL on rat brain membranes. Immunoprecipitation (Vorobiov, 2000; Lee, 2000) Dilutions should be made using a carrier protein such as BSA (1-3%) Optimal working dilutions must be determined by the end user. Research Category Neuroscience Research Sub Category Neurotransmitters & Receptors Physical form Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA and 0.05% sodium azide as a preservative. Reconstitute with 200 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min). Storage and Stability Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. Disclaimer Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. Analysis Note Control CONTROL ANTIGEN: Included free of charge with the antibody is 50 μg of control antigen (lyophilized powder in phosphate buffered saline, pH 7.4, containing 5% sucrose and 0.025% sodium azide). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For positive control, in Western blot using 50 ng of protein per lane. For negative control, preincubate 5-7 μg of fusion protein with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user. Other Notes Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.