Description_x000D_ General description_x000D_ Mutanolysin from Streptomyces globisporus consists of two main lytic enzymes and may be a useful agent for dental caries control._x000D_ Application_x000D_ Mutanolysin has been used in a study to assess lysing and generating protoplasts of dairy streptococci. It has also been used in a study to investigate the conversion of group B streptococci to protoplasts. It provides gentle cell lysis for the isolation of easily degradable biomolecules and RNA from bacteria. It has been used in the formation of spheroplasts for isolation of DNA._x000D_ Provides gentle cell lysis for the isolation of easily degradable biomolecules and RNA from bacteria. It has been used in the formation of spheroplasts for isolation of DNA._x000D_ Packaging_x000D_ 1000, 5000, 10000, 50000 units in glass bottle_x000D_ Biochem/physiol Actions_x000D_ Mutanolysin is an N-acetylmuramidase. Like lysozyme, it is a muralytic enzyme that cleaves the β-N-acetylmuramyl-(1→4)-N-acetylglucosamine linkage of the bacterial cell wall polymer peptidoglycan-polysaccharide. Its carboxy terminal moieties are involved in the recognition and binding of unique cell wall polymers. Mutanolysin lyses Listeria and other Gram-positive bacteria such as Lactobacillus and Lactococcus._x000D_ Unit Definition_x000D_ One unit will produce a ΔA600 of 0.01 per minute at pH 6.0 at 37 °C in a 1 mL volume using a suspension of Streptococcus faecalis cell wall as substrate._x000D_ Physical form_x000D_ Lyophilized powder containing Ficoll® and sodium succinate buffer salts_x000D_ Other Notes_x000D_ View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer_x000D_ Legal Information_x000D_ Fiberfrax is a registered trademark of Unifrax Corp.