Description Specificity Reacts with myelin proteolipid protein in many mammalian species. Shows cross-reactivity to DM20. Immunogen Epitope: C-terminus Myelin proteolipid protein, synthetic peptide CGRGTKF corresponding to the C-terminal peptide. Application Detect Myelin Proteolipid Protein using this Anti-Myelin Proteolipid Protein Antibody, C-terminus, clone PLPC1 validated for use in IH, IH(P) & WB. Research Category Neuroscience Research Sub Category Neuronal & Glial Markers Neurochemistry & Neurotrophins Western Blotting: detects a 23-25kDa protein and DM20 a 20kDa protein; PLP can aggregate causing HMW bands to sometimes appear in certain preparations. Immunocytochemistry: 4% PFA fixed cells, 5-10 minutes room temperature. Use 0.1% triton in block only to allow antibody access to C-terminal epitope. Immunohistology on fresh frozen sections or 4% PFA fixed, frozen tissues. Epitope is internal, use 0.1-0.2% triton X-100 in the blocking step for permeabilization. Immunohistochemistry in paraffin: works best with lightly fixed tissues, or Bouin′s fixed material, although there are reports of traditional formalin fixation also working with antigen recovery. {Mathis, et al 2003}. Optimal working dilutions must be determined by end user. Target description 23-25 kDa Physical form Format: Purified Protein A Purfied Purified mouse immunoglobulin in PBS, pH 7.2 containing 0.09% sodium azide. Storage and Stability Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Disclaimer Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. Linkage Replaces: MAB388 Analysis Note Control Brain Other Notes Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.