Description_x000D_ Application_x000D_ Sequencing primers are used for double-stranded DNA sequencing of inserts in bacterial FLAG expression vectors._x000D_ The N-26 primer is used for sequencing DNA at the 5′ end of the ORF in E. coli FLAG® expression vectors. This corresponds to the amino-terminal fusion junction of FLAG fusion proteins. It can also be used to sequence the amino-terminus of carboxy-terminal FLAG fusion proteins. Complementary sequence is immediately 5′ to the tac promoter region._x000D_ Other Notes_x000D_ (5′-CAT CAT AAC GGT TCT GGC AAA TAT TC-3′)_x000D_ Legal Information_x000D_ FLAG is a registered trademark of Sigma-Aldrich Co. LLC