Description General description Note: 1 mU = 1 milliunit. Recombinant, Chryseobacterium meningosepticum N-Glycosidase F expressed in E. coli. This enzyme from Chryseobacterium (formerly Flavobacterium) meningosepticum catalyzes the hydrolysis of N-glycans from high mannose, hybrid and complex glycopeptides, and from glycoproteins generating asparagine-linked oligosaccharides. Supplied with a series of buffers. Recombinant, Chryseobacterium meningosepticum N-Glycosidase F expressed in E. coli. This enzyme from Chryseobacterium (formerly Flavobacterium) meningosepticum catalyzes the hydrolysis of N-glycans from high mannose, hybrid and complex glycopeptides, and from glycoproteins generating asparagine-linked oligosaccharides. Packaging 1 set in Glass bottle Warning Toxicity: Standard Handling (A) Unit Definition One unit is defined as the amount of enzyme that will completely catalyze the release of N-linked oligosaccharides from 1 µmol denatured ribonuclease in 1 min at 37°C, pH 7.5. Physical form Enzyme supplied in 50 mM NaCl, 20 mM Tris-HCl, 1 mM EDTA, pH 7.5. Reconstitution Following initial thaw, aliquot and freeze (-20°C). Other Notes Fan, J.-Q., and Lee, Y.C. 1997. J. Biol. Chem. 272, 27058. Altmann, F., et al. 1995. Glycoconj. J. 12, 84.; Description