Description_x000D_ Application_x000D_ N-p-Tosyl-L-phenylalanine chloromethyl ketone has been used:_x000D_ • to investigate whether transcription factor NFκB is involved in renalase expression_x000D_ • to test the role of NF-κB activation_x000D_ • to study its inhibitory effect on chymotrypsin_x000D_ Irreversible inhibitor of certain serine proteases; used to inhibit chymotrypsin in preparations of trypsin._x000D_ Biochem/physiol Actions_x000D_ Blocks the LPS- or cytokine-induced activation of nuclear factor κB (NFκB), which, in turn, blocks the induction of iNOS and COX-2 transcription. Blocks activation of pp70s6k by all mitogens. Blocks apoptosis cell lines by inhibiting the processing of caspases in some cell lines and to some stimuli. Also blocks apoptosis initiated by Taxol (in MCF-7 human breast carcinoma cells)._x000D_ N-p-Tosyl-L-phenylalanine chloromethyl ketone (TPCK) blocks the lipopolysaccharide (LPS)- or cytokine-induced activation of nuclear factor κB (NFκB), which, in turn, blocks the induction of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) transcription. Blocks activation of pp70s6k by all mitogens. Blocks apoptosis cell lines by inhibiting the processing of caspases in some cell lines and to some stimuli. Also blocks apoptosis initiated by Taxol (in MCF-7 human breast carcinoma cells). TPCK recognises the phenylalanine residue in chymotrypsin′s and prevents its function._x000D_ Features and Benefits_x000D_ This compound is also offered as part of Sigma′s Library of Pharmacologically Active Compounds (LOPAC®1280), a biologically annotated collection of high-quality, ready-to-screen compounds. Click here to learn more._x000D_ Packaging_x000D_ Bottomless glass bottle. Contents are inside inserted fused cone._x000D_ Caution_x000D_ Shelf-life is at least two years when stored desiccated at -20 °C._x000D_ Legal Information_x000D_ LOPAC is a registered trademark of Sigma-Aldrich Co. LLC