Description_x000D_ General description_x000D_ Naphthol AS-BI (7-Bromo-3-hydroxy-2-naphtho-o-anisidine) is a substituted naphthol._x000D_ Application_x000D_ Naphthol AS-BI phosphate disodium salt hydrate is suitable to use:_x000D_ • in tartrate-resistant acid phosphatase (TRAP) staining to detect osteoclast activity_x000D_ • as a component in Tris-HCl in staining cells to measure alkaline phosphatase (ALP) activity_x000D_ • to perform alkaline phosphatase reaction_x000D_ Biochem/physiol Actions_x000D_ Naphthol AS-BI (7-Bromo-3-hydroxy-2-naphtho-o-anisidine) can be used as a substrate to determine alkaline and acid phosphatase. It is also used to determine the activity of acid phosphatase in single cells._x000D_ Substrates_x000D_ Substrate for the histochemical demonstration of acid and alkaline phosphatase.