Description_x000D_ Application_x000D_ Useful for kinase assay and Western blot._x000D_ Biochem/physiol Actions_x000D_ Recombinant human NEK8 (1-322) was expressed by Baculovirus in Sf9 insect cells using an N-terminal GST tag. NEK8 or NIMA-related kinase 8 is a member of the serine/threionine protein kinase family related to NIMA (never in mitosis, gene A) of Aspergillus nidulans which may play a role in cell cycle progression from G2 to M phase. NEK8 is part of a complex of ciliary proteins required for renal and cardiovascular development (1). NEK8 interacts with polycystin signal transduction pathways and may control the targeting of these ciliary proteins (2)._x000D_ Reconstitution_x000D_ Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol._x000D_ Storage and Stability_x000D_ Store in aliquots at -70°C. Avoid freeze/thaw cycles._x000D_ Legal Information_x000D_ PRECISIO is a registered trademark of Sigma-Aldrich Co. LLC