Description_x000D_ General description_x000D_ Nerve growth factor (NGF) is a dimer of 118-residue polypeptide chains linked non-covalently. Both these polypeptides contain three intramolecular disulphide bridges. It forms the prototype of the neurotrophin growth factor family. In male mouse submandibular salivary gland, NGF is present in high amounts, and is present as a high molecular weight complex called nerve growth factor-7S (NGF-7S). NGF-7S is an α2β2γ2 complex, where β2-NGF is the active neurotrophin, and is linked to the dimers of the glandular kallikrein serine proteinases members, α-NGF and γ-NGF._x000D_ Application_x000D_ Nerve Growth Factor-7S (NGF-7S) from murine submaxillary gland has been used for the treatment of following cells to induce neuronal differentiation-_x000D_ • PC12_x000D_ • Dorsal root ganglia (DRG) obtained from chick embryos of either sex (Gallus gallus)_x000D_ Biochem/physiol Actions_x000D_ Nerve growth factor (NGF) is a neurotrophin, which facilitates the differentiation and survival of specific subtypes of neurons of central nervous system and peripheral nervous system. However, nerve growth factor-7S (NGF-7S) lacks any neurotrophic activity as most part of β-NGF subunit is buried within the NGF-7S complex._x000D_ Physical form_x000D_ Lyophilized from 0.2 μm filtered solution in 25 mM sodium phosphate, pH 7.0._x000D_ Preparation Note_x000D_ Nerve growth factor-7S (NGF-7S) is isolated from male mouse submaxillary glands using a modification of the method of Varon, et al._x000D_ Analysis Note_x000D_ The biological activity is measured using a 3-day MTT assay.