Description_x000D_ General description_x000D_ This Neutralization Buffer B is intended for use with Sigma′s REDExtract-N-Amp™ Tissue PCR Kits. This buffer is used to neutralize the DNA extract and be combined with the REDExtract-N-Amp PCR Reaction Mix and PCR primers to amplify target DNA. The REDExtract-N-Amp Kits include a sufficient volume of Neutralization Buffer B for at least one neutralization per extraction. However, where additional amplifications are required, supplemental Neutralization Buffer may be needed._x000D_ Application_x000D_ Neutralization Solution B has been used in the modified Sigma Extract-N-Amp extraction procedure. It has also been used in genotyping of mice, for the treatment of samples prior to polymerase chain reaction (PCR) using Sigma′s REDExtract-N-Amp PCR Reaction Mix._x000D_ Suitable for neutralizing the DNA extract obtained with use of Sigma′s REDExtract-N-Amp™ Tissue PCR Kits._x000D_ Packaging_x000D_ 24 mL in poly bottle_x000D_ Legal Information_x000D_ REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC