Description_x000D_ Application_x000D_ Phthaldialdehyde Reagent has been used for the derivatization plasma samples prior to high performance liquid chromatography._x000D_ Precolumn derivatization reagent for primary amines and amino acids. The fluorescent derivative can be detected by reverse-phase HPLC. The reaction requires OPA, primary amine and a sulfhydryl. In the presence of excess sulfhydryl, amines can be quantitated. In the presence of excess amine, sulfhydryls can be quantitated._x000D_ Linkage_x000D_ Same as P 0532, but contains no 2-mercaptoethanol.