Description General description Single chain vitamin K-dependent protein produced by osteoblasts. Osteocalcin purified from bovine bone. Binds to phospholipid vesicles in the presence of Ca2+ (Kd = 6 µM). Also binds hydroxylapatite. A good marker of bone turnover. May play a role in bone mineralization, bone resorption, and bone formation. Single chain vitamin K-dependent protein produced by osteoblasts. Osteocalcin purified from bovine bone. Binds to phospholipid vesicles in the presence of Ca2+ (Kd = 6 µM). Also binds hydroxylapatite. Possible role in bone mineralization, bone resorption, and bone formation. Note: Gla corresponds to γ-carboxyglutamic acid. Packaging 100 μg in Plastic ampoule Warning Toxicity: Standard Handling (A) Sequence H-Tyr-Leu-Asp-His-Trp-Leu-Gly-Ala-Pro-Ala-Pro-Tyr-Pro-Asp-Pro-Leu-Gla-Pro-Lys-Arg-Gla-Val-Cys²³-Gla-Leu-Asn-Pro-Asp-Cys²⁹-Asp-Glu-Leu-Ala-Asp-His-Ile-Gly-Phe-Gln-Glu-Ala-Tyr-Arg-Arg-Phe-Tyr-Gly-Pro-Val-OH (disulfide bond: 23 → 29) Physical form In 75 mM NaCl, 10 mM sodium phosphate buffer, pH 7.4. Reconstitution Following initial thaw, aliquot and freeze (-20°C). Other Notes Carter, S.D., et al. 1996. J. Anim. Sci. 74, 2719. Watson, K.E., et al. 1994. J. Clin. Invest.93, 2106. Tracy, R.P., et al. 1990. J. Bone Min. Res.5, 451. Gendreau, M.A., et al. 1989. J. Biol. Chem.264, 6972.