Description General description Alternative to DFP and PMSF. An irreversible inhibitor of trypsin-like serine proteases. Inhibits trypsin and thrombin in equimolar concentration. Acts on C1r, C1s, Factor Xa, and plasmin, requiring a 5- to 10-fold excess for complete irreversible inhibition. Inhibitory activity is approximately 1000-fold greater than that of PMSF. An alternative to DFP and PMSF. Specific irreversible inhibitor of trypsin-like serine proteases. Inhibits trypsin and thrombin in equimolar concentrations. Acts on C1r, C1s, Factor Xa and plasmin, requiring a 5- to 10-fold excess for complete irreversible inhibition. Inhibitory activity is approximately 1000-fold greater than that of PMSF. Stability is pH dependent: t½ = 20 min at pH 6.0; 6 min at pH 7.0; 1 millisecond at pH 8.0. Packaging 5 mg in Plastic ampoule Biochem/physiol Actions Cell permeable: no Primary Target trypsin-like serine proteases Product does not compete with ATP. Reversible: no Warning Toxicity: Standard Handling (A) Reconstitution Unstable in solution; reconstitute just prior to use. Other Notes Cole, T.C., et al. 1989. Biochim. Biophys. Acta 990, 254. Laura, R., et al. 1980. Biochemistry19, 4859.