Description_x000D_ General description_x000D_ Sigma’s pBR322 Hae III Digest contains 22 fragments from 8 - 587 bp. Ten ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel._x000D_ Application_x000D_ Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels._x000D_ Components_x000D_ Sigma’s pBR322 Hae III Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA._x000D_ Other Notes_x000D_ Contains 22 fragments, 8–587 bp_x000D_ For optimal resolution, the recommended agarose gel concentration is 2.5._x000D_ Preparation Note_x000D_ Plasmid pBR322 DNA was digested with Hae III to completion, then purified by phenol extraction.