Description_x000D_ General description_x000D_ We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in cellulosic ethanol research. For more information see the article in biofiles and Enzymes for Alternative Energy Research._x000D_ Application_x000D_ Petctinase from Aspergillus niger has been used:_x000D_ • for protoplasts liberation_x000D_ • in the protoplast enzyme mixture for enzyme digestion_x000D_ • to conduct partial saccharification of sugars_x000D_ • to study their role in the invasion of plant tissues by phytopathogens, the spoilage of produce and various food processing and plant biotechnology applications_x000D_ Used in plant protoplast preparation to digest cell wall prior to organelle isolation._x000D_ Packaging_x000D_ 25000, 100000 units in glass bottle_x000D_ Biochem/physiol Actions_x000D_ Pectolytic enzyme preparation produced from a selected strain of Aspergillus niger: contains mainly pectintranseliminase, polygalacturonase, and pectinesterase and small amounts of hemicellulases and cellulases. Pectinases hydrolyses pectin, which is a component of the cell wall. They may attack methyl-esterified pectin or de-esterified pectin. It is a source of pectinase activity, also containing cellulase and hemicellulase activities.Pectinase catalyzes the random hydrolysis of a-(1-4)-Dgalactosiduronic linkages in pectin and other galacturonans._x000D_ Unit Definition_x000D_ One unit will liberate 1.0 μmole of galacturonic acid from polygalacturonic acid per min at pH 4.0 at 25 °C._x000D_ Physical form_x000D_ Solution in 40% glycerol_x000D_ Other Notes_x000D_ View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer