Description_x000D_ General description_x000D_ We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in cellulosic ethanol research. For more information see the article in biofiles and Enzymes for Alternative Energy Research._x000D_ Pectolyase or pectin lyase is stable in the temperature range of 40-50°C and is a 50 kDa enzyme with an isoelectric pH of 3.8._x000D_ Application_x000D_ Pectolyase from Aspergillus japonicus has been used:_x000D_ • in the lysis of chlorella cells for protoplast generation_x000D_ • as component of enzyme digestion buffer for Arabidopsis meiotic chromosome isolation_x000D_ • to induce reorganization of Arabidopsis root cap cell architecture_x000D_ Used in plant protoplast preparation to digest cell wall prior to organelle isolation. It is used in complex carbohydrate analysis. Product P3026 is provided in a lyophilized powder containing lactose. Product P3026 has been used to digest Crassula root tips prior to meiotic analysis._x000D_ Used in plant protoplast preparation to digest cell wall prior to organelle isolation._x000D_ Packaging_x000D_ 1 g in poly bottle_x000D_ 100, 250 mg in poly bottle_x000D_ Biochem/physiol Actions_x000D_ Pectolyase catalyzes the eliminative cleavage of a-(1- 4)-Dgalacturonan methyl ester to give oligosaccharides with 4-deoxy-6-O-methyl-a-D-galact-4-enuronosyl groups at their non-reducing ends. It contain two types of pectinase, endopolygalacturonase, endo-pectin lyase and a maceration stimulating factora._x000D_ Pectolyase is used for protoplast isolation from plant cells. It also mediates ethylene hormone synthesis._x000D_ Other Notes_x000D_ Reported to contain two types of pectinase, endopolygalacturonase (EC3.2.1.15), endopectin lyase (EC4.2.2.10) and a maceration stimulating factor._x000D_ View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer_x000D_ Unit Definition_x000D_ One unit will liberate 1.0 μmole of galacturonic acid from polygalacturonic acid per min at pH 5.5 at 25 °C.