Description_x000D_ Application_x000D_ PEG is used as a fusogen to obtain hybridomas for monoclonal antibody production. Induces cell hybridization._x000D_ Recommended for use in a normal fusion protocol requiring 50% PEG and 10% DMSO._x000D_ Packaging_x000D_ Packaged in sealed ampules under nitrogen._x000D_ Other Notes_x000D_ Contains 50% (w/v) polyethylene glycol (Av. Mol. Wt. 1450) and 10% DMSO (v/v) in DPBS without Calcium._x000D_ Reconstitution_x000D_ Solution is ready-to-use. If a less concentrated solution is desired, dilute with sterile DPBS without calcium (D5773). Some precipitate may appear after being exposed to cooler, but should disappear as the solution warms. Solution may be frozen if desired but should first be aliquotted to avoid repeated freeze/thaw cycles._x000D_ Legal Information_x000D_ Hybri-Max is a trademark of Sigma-Aldrich Co. LLC