Description_x000D_ Packaging_x000D_ 5, 10, 100 g in glass bottle_x000D_ Application_x000D_ Pepsin cleavage can be used to produce F(ab')2 fragments of antibodies. pepsin at www.sigma-aldrich.com/enzymeexplorer._x000D_ Biochem/physiol Actions_x000D_ Preferential cleavage: hydrophobic and aromatic residues in P1 and P1′ postitions. Cleaves Phe-Val, Gln-His, Glu-Ala, Ala-Leu, Leu-Tyr, Tyr-Leu, Gly-Phe, Phe-Phe and Phe-Tyr bonds in the β chain of insulin_x000D_ Unit Definition_x000D_ One unit will produce a ΔA280 of 0.001 per min at pH2.0 at 37° C, measured as TCA-soluble products using hemoglobin as substrate. (Final volume = 16mL. Light path = 1cm.)_x000D_ Analysis Note_x000D_ Optimum pH is 2-4. Active in 4 M urea and 3 M guanidine HCl. Stable at 60 °C. Pepsin is irreversibly inactivated at pH 8.0 - 8.5._x000D_ Protein determined by E1%/280_x000D_ Other Notes_x000D_ View more information on pepsin at www.sigma-aldrich.com/enzymeexplorer.