Description_x000D_ General description_x000D_ Sigma’s PerfectHyb Plus is a ready-to-use hybridization buffer for Northern and Southern blot protocols. Use of PerfectHyb Plus can decrease hybridization time to as little as two hours._x000D_ Application_x000D_ Suitable for use in hybridization buffers used in Northern blot, Southern blot, colony/plaque blots, and dot blots._x000D_ Features and Benefits_x000D_ • Fast and easy-to-use_x000D_ • Up to 20x more sensitive than conventional buffers_x000D_ • Doesn’t require blocking steps_x000D_ • Compatible with neutral and positively-charged membranes_x000D_ • Compatible with chemiluminescent, colorimetric , and DIG detection assays_x000D_ • Compatible with radioactive and non-radioactive probes_x000D_ • Compatible with single or double-stranded DNA, RNA, and oligonucleotide probes_x000D_ Other Notes_x000D_ A small amount of precipitate that has formed in the solution can be dissolved at 30-35°C with occasional gentle inversion._x000D_ Legal Information_x000D_ PerfectHyb is a trademark of Sigma-Aldrich Co. LLC