Description_x000D_ General description_x000D_ Peroxidase (POD) is an enzyme that includes a group of specific enzymes such as nicotinamide adenine dinucleotide (NAD) peroxidase, NADP peroxidase, fatty acid peroxidase and a group of non-specific enzymes from various sources. It is present in animals, higher plants and other organisms._x000D_ Application_x000D_ For direct conjugation to compounds containing free sulfhydryl groups for use as an enzyme-linked detection reagent._x000D_ Instructions for use included. In a study on horseradish peroxidase, the Maleimide compounds succinimidyl 4-maleimidobutyrate and succinimidyl 6-maleimidohexanoate have been shown to be the most effective in the conjugation of Fab′ to horseradish peroxidase. This enzyme has also been used in a study to investigate the effectiveness in staining a mitochondrial protein, lipoamide dehydrogenase._x000D_ To increase signal intensity of immunoconjugates, immunoglobulins were synthetically modified to contain multiple primary amines and covalently coupled to maleimide activated peroxidase. This can result in more efficient conjugation and signal amplification._x000D_ Biochem/physiol Actions_x000D_ Peroxidase (POD) helps in the dehydrogenation of several organic compounds like phenols, aromatic amines, hydroquinones etc._x000D_ Other Notes_x000D_ Type VI peroxidase activated with maleimidocaproic acid N-hydroxysuccinimide ester._x000D_ Unit Definition_x000D_ One unit will form 1.0 mg of purpurogallin from pyrogallol in 20 seconds at pH 6.0 at 20°C._x000D_ Physical form_x000D_ Lyophilized powder containing trehalose and sodium citrate