Description_x000D_ Application_x000D_ Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It may be used for protein labeling when high sensitivity is required. Product P4252 has been used for bulk digestion of tRNA . Product P4252 is provided in the ratio of 30-90 units/mg of protein._x000D_ Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It may be used for protein labeling when high sensitivity is required. Product P4252 is an ammonium sulfate suspension in glycine buffer._x000D_ Packaging_x000D_ 100, 200, 1000 units in glass bottle_x000D_ Biochem/physiol Actions_x000D_ Alkaline phosphatase, from Escherichia coli, is a dimeric, non-glycosylated protein which mainly reside in the periplasmic space. Three known isoforms exist. The enzyme requires zinc, and is activated by magnesium. E. coli akaline phosphatase has a broad specificity for phosphate esters._x000D_ Alkaline phosphatase, from Escherichia coli, is a dimeric, non-glycosylated protein which mainly reside in the periplasmic space. Three known isoforms exist. The enzyme requires zinc, and is activated by magnesium. E. coli akaline phosphatase has a broad specificity for phosphate esters._x000D_ Caution_x000D_ Presence of 0.1% SDS in the alkaline phosphatase assays will inhibit any nuclease contamination in the sample._x000D_ Unit Definition_x000D_ One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 10.4 at 37 °C._x000D_ Physical form_x000D_ Suspension in 2.5 M (NH4)2SO4_x000D_ Analysis Note_x000D_ The activity at pH 8.0 at 25 °C is approximately the same as at pH 10.4 at 37 °C.