Description_x000D_ General description_x000D_ Phosphatidylcholine (PC) accounts for more than 50% of the phospholipids composing mammalian plasma membranes and is particularly enriched in the extracellular leaflet. PC is synthesized in the liver and is required for lipoprotein assembly and secretion. PC is cleaved by phospholipase D, which hydrolyses the choline head, producing the signaling intermediate phosphatidic acid._x000D_ Application_x000D_ Phosphatidylcholine Assay Kit has been used to determine the concentration of phosphatidylcholine in samples._x000D_ Suitability_x000D_ Suitable for the measurement of phosphatidylcholine in a variety of biological samples_x000D_ Principle_x000D_ In this assay, PC concentration is determined by a coupled enzyme reaction, which results in a colorimetric (570 nm)/fluorometric (λex = 535/λem = 587 nm) product, proportional to the PC present.