Description General description Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~44 kDa PDH-E1α protein phosphorylated at Ser232. Recognizes the ~44 kDa PDH-E1α protein phosphorylated at Ser232 in HEK293 cells. This PhosphoDetect Anti-PDH-E1α (pSer²³²) Rabbit pAb is validated for use in Immunoblotting, IF, IP for the detection of PDH-E1α (pSer²³²). Immunogen Human a synthetic phosphopeptide corresponding to amino acids surrounding the Ser²³² phosphorylation site of human PDH-E1α Application Immunoblotting (0.25 µg/ml) Immunofluorescence (Please see comments) Immunoprecipitation (1 µg/ml) Physical form In PBS. Reconstitution Following initial thaw, aliquot and freeze (-20°C). Packaging 50 μg in Plastic ampoule Warning Toxicity: Standard Handling (A) Analysis Note Positive Control HEK293 cells Other Notes Rardin M.J., et. al. 2009. Anal. Biochem.2, 157. Seifert, F., et al. 2007. Biochemistry 21, 6277. Lee, J., et al. 2007. Mol. Cell Prot. 4, 669. Patel, M.S. and Korotchkina, L.G. 2006 Biochem. Soc. Trans.34, 217. Korotchkina, L.G., et al. 2001. J. Biol. Chem. 40, 37223. This antibody has been known to work with immunofluorescence. Antibody should be titrated for optimal results in individual systems.