Description General description Protein A and immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~72 kDa Syk protein phosphorylated on Tyr525/526. Recognizes the ~72 kDa Syk protein phosphorylated at Tyr525/526 in human Syk or Tyr519/520 in mouse Syk in 293T cells expressing wild-type Syk and CD8. This PhosphoDetect Anti-Syk (pTyr525/526) Rabbit pAb is validated for use in ELISA, Immunoblotting for the detection of Syk (pTyr525/526). Immunogen Human a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr525/526 phosphorylation sites of human Syk Application ELISA (1:1000) Immunoblotting (1:1000) Physical form In 150 mM NaCl, 10 mM sodium HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5. Reconstitution Do not aliquot. Legal Information TWEEN is a registered trademark of Croda International PLC Packaging 50 μL in Plastic ampoule Warning Toxicity: Standard Handling (A) Analysis Note Positive Control 293T cells transfected with wild-type Syk and CD8 Other Notes Coopman, P.J., et al. 2000. Nature406, 742. Turner, M., et al. 2000. Immunol. Today21, 148. Zang, J., et al. 2000. J. Biol. Chem.275, 35442. Chu, D.H., et. al. 1998. Immunol. Rev.165, 167. Cheng, A.M. and Chan, A.C. 1997. Curr. Opin. Immunol.9, 528. Kurosaki, T. 1997. Curr. Opin. Immunol.9, 309. Tyr525/526 represent the phosphorylation sites in human Syk, while Tyr519/520 are the equivalent phosphorylation sites in mouse Syk. For immunoblotting the membrane should be incubated with antibody diluted in 5% BSA, 1X TBS, 0.1% Tween®-20 detergent at 4°C overnight with gentle shaking. Antibody should be titrated for optimal results in individual systems.