Description_x000D_ General description_x000D_ Phosphodiesterase (PDE) II belong to the PDE superfamily and includes subtypes from one to 11. They exists as a dimer and comprise a N-terminal regulatory domain, C-terminal prenylated domain and a Zn2+ containing catalytic domain. The bovine spleen PDE2 corresponds to a molecular weight of 65 kDa. It requires divalent cations for its enzymatic activity and has an optimum pH of 7.5._x000D_ Application_x000D_ Phosphodiesterase II from bovine spleen has been used in the:_x000D_ • excision of pyridyloxobutyl (POB) base adducts from DNA_x000D_ • digestion of DNA prior to cycloadenosine enrichment_x000D_ • hydrolysis of DNA from blue mussels gill tissue into deoxyribonucleoside 3′-monophosphates_x000D_ Packaging_x000D_ 10 units in glass bottle_x000D_ 25 units in poly bottle_x000D_ Biochem/physiol Actions_x000D_ Phosphodiesterase (PDE) breaks phosphodiester bonds. 3-Isobutyl-methylxanthine (IBMX) is a potential PDE2 inhibitor._x000D_ The enzyme acts on poly(A), poly(U), and poly(I). Native DNA and poly(C) are quite resistant to the action of this enzyme._x000D_ Hydrolyzes RNA, RNA-Core, 3′-alkyl- and 3′-aryl-nucleoside phosphates, and polydeoxyribonucleotides with 3′-phosphate end groups to 3′-mononucleotides._x000D_ Polynucleotides having 5′-phosphomonoester end groups are not attacked._x000D_ Unit Definition_x000D_ One unit will produce acid soluble nucleotides equivalent to a ΔA260 of 16 in 30 min at pH 6.5 at 37 °C, in a 2.0 mL reaction mixture. Substrate: RNA-Core. Actual A260 is measured on the supernatant after precipitation of the unhydrolyzed RNA with uranyl acetate-perchloric acid reagent._x000D_ Analysis Note_x000D_ Protein determined by biuret