Description General description Recombinant, bovine c-GMP-specific phosphodiesterase expressed in S. frugiperda insect cells. Supplied as partially purified, catalytically active, dimeric holoenzyme. A target enzyme for Viagra (sildenafil). Suitable for enzymatic studies and as a standard in Western blots using Anti-Phosphodiesterase V Antibody (Cat. No. 524583). For enzymatic studies a dilution of 1:1000 recommended. For use as a immunoblot standard 2 to 3 µl per lane is recommended. In immunoblotting, gives band at ~92 kDa. Recombinant, bovine c-GMP-specific phosphodiesterase expressed in S. frugiperda insect cells. Supplied as partially purified, catalytically active, dimeric holoenzyme. A target enzyme for Viagra (sildenafil). Suitable for enzymatic studies and as a standard in Western blots using Anti-Phosphodiesterase V Antibody (Cat. No. 524583). Packaging 50 μL in Plastic ampoule Warning Toxicity: Standard Handling (A) Unit Definition One unit is defined as the amount of enzyme that will hydrolyze 1.0 pmol cGMP per min at 25°C, pH 7.2. Physical form In sodium phosphate buffer, 20% glycerol, pH 7.2. Reconstitution Following initial thaw, aliquot and freeze (-70°C). Other Notes Gibson, L.C.D., et al. 2006. Eur. J. Pharmacol.538, 39. Webb, D.J., et al. 1999. Am. J. Cardiol. 83, 21C. Turko, I.V., et al. 1998. J. Biol. Chem. 273, 6460.