Description_x000D_ General description_x000D_ Phospholipase A2 from bovine pancreas is a calcium-dependent lipolytic enzyme._x000D_ Application_x000D_ Phospholipase A2 from bovine pancreas has been used to determine rat renal proximal tubular segments (PTS) viability during oxygenation and hypoxia-reoxygenation and to study the impact of phospholipase A2 on tubular cell triglyceride levels._x000D_ Packaging_x000D_ 10, 50 mg in poly bottle_x000D_ 5 mg in glass bottle_x000D_ Package size based on protein content_x000D_ Biochem/physiol Actions_x000D_ Hydrolyzes the β-ester bond of zwitterionic glycerophospholipids. Preferred substrates are phosphatidylcholine, phosphatidylethanolamine, and their plasmalogen analogues. Phosphatidylinositol and phosphatidylserine are also hydrolyzed. Aggressively attacks phospholipids in membranes of intact cells._x000D_ Unit Definition_x000D_ One unit will hydrolyze 1.0 μmole of soybean L-α-phosphatidylcholine to L-α-lysophosphatidylcholine and a fatty acid per min at pH 8.0 and 37 °C._x000D_ Physical form_x000D_ Contains Tris buffer salts._x000D_ Analysis Note_x000D_ Protein determined by biuret.