Description_x000D_ General description_x000D_ Phosphatidylinositol-specific phospholipase C (PI-PLC) from Bacillus cereus is a 298 residual protein. It belongs to the class of ubiquitous enzymes. PI-PLC is characterized with a single globular domain and it folds into eight (βα) barrel sheets, out of which only six βα units are found in barrel, lacking helices between strands IV and V and V and VI._x000D_ Application_x000D_ Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus has been used to cleave GPI-anchored protein from green fluorescent protein- glycosylphosphatidylinositol (GFP-GPI) from the cell membrane. It has also been used in invasion assay to test the involvement of PLAP in T. cruzi invasion to human epithelial type 2 (HEp-2) cells._x000D_ Packaging_x000D_ 5, 25 units in glass bottle_x000D_ Biochem/physiol Actions_x000D_ Used for the release of GPI anchored proteins from the membrane._x000D_ Unit Definition_x000D_ One unit will liberate one unit of acetylcholinesterase per minute from a membrane-bound crude preparation at pH 7.4 at 30 °C (10 minute incubation)._x000D_ Physical form_x000D_ Contains phosphate buffer salts, EDTA and stabilizer_x000D_ Analysis Note_x000D_ Acetylcholinesterase is measured according to Ellman, et al.