Description_x000D_ General description_x000D_ Norgen Biotek′s Plant/Fungi Total RNA Purification Kit provides a rapid method for the isolation and purification of total RNA, including viral RNA, from a wide range of plant and filamentous fungal species. Total RNA can be purified from fresh or frozen plant tissues, plant cells or filamentous fungi samples using this kit. All sizes of RNA are purified, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The procedure is rapid and convenient, as it does not rely on the use of liquid nitrogen in order to homogenize the samples._x000D_ Application_x000D_ For the rapid purification of total RNA (including microRNA) from plants and fungi._x000D_ Purified RNA is of the highest quality and can be used in a number of downstream applications, including:_x000D_ • qRT-PCR_x000D_ • Reverse transcription PCR_x000D_ • Northern blotting_x000D_ • RNAse protection_x000D_ • Primer extension_x000D_ • Expression array assays_x000D_ • Next Generation Sequencing_x000D_ Features and Benefits_x000D_ • Isolate RNA from a wide range of plant and filamentous fungi samples._x000D_ • Robust lysis buffer is suitable for challenging samples such as pine needle or grape leaf._x000D_ • Isolate a diversity of RNA species from large mRNA, microRNA, and viral RNA._x000D_ • Purification of high yield and quality RNA._x000D_ • No phenol/chloroform extraction required._x000D_ • Liquid nitrogen is not required for homogenization_x000D_ Other Notes_x000D_ Storage Conditions and Product Stability_x000D_ All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers._x000D_ We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.