Description General description Native Glu-type plasminogen from human plasma. Plasminogen is a single chain glycoprotein found in normal plasma at ~12 mg per deciliter, and is the inactive precursor of the serine protease plasmin. Native Glu-type plasminogen from human plasma. Plasminogen is a single chain glycoprotein zymogen, which is synthesized in the liver and circulates in plasma at a concentration of approximately 2.4 µM. The plasminogen molecule contains 790 amino acids, 24 disulfide bridges, no free sulfhydryls, and 5 regions of internal sequence homology, known as kringles, between Lys77 and Arg560. These kringle regions are homologous to the kringle domains in t-PA, u-PA, and prothrombin. Native Glu-plasminogen is readily converted to Lys77-plasminogen by plasmin hydrolysis of the Lys76-Lys77 peptide bond. The plasminogen is converted to plasmin by cleavage at Arg540-Val561 by urokinase, streptokinase, or tissue plasminogen activator. Packaging 1 mg in Plastic ampoule Warning Toxicity: Irritant (B) Physical form In 100 mM NaCl, 50 mM Tris-HCl, pH 7.5. Preparation Note Prepared from plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV. Reconstitution Following initial thaw, aliquot and freeze (-20°C). Analysis Note Single band by SDS-PAGE Other Notes Collen, D. 1986. In Blood Coagulation (Zwaal, R.E.A., and Hemker, H.C. eds.) Elsevier, N.Y. p. 243. Barlow, G.H., et al. 1984. Biochemistry23, 2384. Wohl, R.C., et al. 1982. Thromb. Res.27, 523. Castellino, F.J., et al. 1981. Methods Enzymol.80, 365. Robbins, K.C. 1976. Methods Enzymol.45, 257.