Description Specificity Recognizes Nav1.7 protein (PN1, NENA, NE, Type IX Voltage Gated Sodium Channel, Scn9a). The epitope is specific for Nav1.7 and is not present in any other known protein. SPECIES REACTIVITIES: The immunogen sequence is highly conserved in rabbit and human (14/15 and 13/15 respectively). Other species have not been tested. Immunogen Purified peptide from rat Nav1.7 (amino acids 446-460) (Accession AAB50403). Application Detect Sodium Channel Nav1.7 using this Anti-Sodium Channel Nav1.7 Antibody, Pain validated for use in IH & WB. Research Category Neuroscience Research Sub Category Ion Channels & Transporters Western blot: 1:200 using ECL on rat brain membranes. Immunohistochemistry on rat dorsal root ganglion (DRG) frozen sections. Dilutions should be made using a carrier protein such as BSA (1-3%) Optimal working dilutions must be determined by the end user. Physical form Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, and 0.05% sodium azide as a preservative. Reconstitute with 50 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min). Storage and Stability Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. Disclaimer Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. Analysis Note Control CONTROL ANTIGEN: Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user. Other Notes Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.