Description_x000D_ Application_x000D_ PNGase F from Elizabethkingia meningoseptica has been used in deglycosylation_x000D_ • of recombinant soybean agglutinin (rSBA) in Nicotiana benthamiana (NbrSBA) and Solanum tuberosum (StrSBA)_x000D_ • of frontal cortical lysate to verify the glycosylation profile of β-secretase (BACE proteins)_x000D_ • of cell lysate for evaluating the siRNA silencing of cellular prion protein (PrPc) post transfection_x000D_ PNGase F is a glycosylasparaginase used to deglycosylate proteins. It is widely used in structure-function studies of glycoproteins._x000D_ Used to deglycosylate protein._x000D_ Biochem/physiol Actions_x000D_ PNGase F cleaves an entire glycan from a glycoprotein provided the glycosylated asparagine moiety is substituted on its amino and carboxyl terminus with a polypeptide chain. It deaminates the asparagine to aspartic acid, but leaves the oligosaccharide intact. PNGase F will not remove oligosaccharides containing α(1-3)-linked core fucose, commonly found in plant glycoproteins. A tripeptide with the oligosaccharide-linked asparagine as the central residue is the minimal substrate for PNGase F._x000D_ Cleaves an entire glycan from a glycoprotein provided the glycosylated asparagine moiety is substituted on its amino and carboxyl terminus with a polypeptide chain._x000D_ Packaging_x000D_ Each set includes enzyme, two formulations of 5× reaction buffer (for routine and mass spectrometry downstream analysis), detergent and denaturation solutions_x000D_ Unit Definition_x000D_ One unit will catalyze the release of N-linked oligosaccharides from 1 micromole of denatured ribonuclease B in one minute at 37°C at pH 7.5 monitored by SDS-PAGE. One Sigma unit of PNGase F activity is equal to 1 IUB milliunit._x000D_ Legal Information_x000D_ N-Glycanase is a registered trademark of ProZyme, Inc.