Description_x000D_ General description_x000D_ PNGase F (Peptide N-glycosidase F) cleaves asparagine-linked glycoproteins, which produces carbohydrate-free peptides and detached full-length oligosaccharides._x000D_ Application_x000D_ PNGase F from Elizabethkingia miricola has been used for the deglycosylation of the proteins._x000D_ Used to deglycosylate protein._x000D_ Biochem/physiol Actions_x000D_ Cleaves an entire glycan from a glycoprotein provided the glycosylated asparagine moiety is substituted on its amino and carboxyl terminus with a polypeptide chain._x000D_ Unit Definition_x000D_ One unit will catalyze the release of N-linked oligosaccharides from 1 nanomole of denatured ribonuclease B in one minute at 37°C at pH 7.5 monitored by SDS-PAGE. One Sigma unit of PNGase F activity is equal to 1 IUB milliunit._x000D_ Physical form_x000D_ Supplied as a solution in 20 mM Tris HCl, pH 7.5, 50 mM NaCl and 1 mM EDTA