Description_x000D_ General description_x000D_ Prolidase is a cytosolic exopeptidase. It is a homodimeric enzyme which requires divalent cations like manganese as a cofactor in its active site for its function._x000D_ Application_x000D_ Prolidase from porcine kidney has been used:_x000D_ • in the enzymatic hydrolysis of porcine milk for the recovery of L-glutamine from proteins and peptides_x000D_ • in the proteolysis of skim milk for the determination of ε-(γ-glutamyl)lysine and free aminoacids_x000D_ • to determine its effect on the activity of enterococcin A 2000_x000D_ Prolidase has an important role in recycling of proline and collagen production. It is used to study mutations in the PEPD gene that cause prolidase deficiency. It is used to hydrolyze proteins with C-terminal proline or hydroxyproline residues. Prolidase, product P6675 from porcine kidney, has been used to hydrolyze peptide bonds from the amino terminus when studying enzymatic methylation of membrane proteins._x000D_ Packaging_x000D_ 250, 1000 units in glass bottle_x000D_ Biochem/physiol Actions_x000D_ Prolidase is an enzyme that catalyzes the hydrolysis of the imide bond between an α-carboxyl group and proline or hydroxyproline. The protein forms a homodimer that hydrolyzes dipeptides or tripeptides with C-terminal proline or hydroxyproline residues._x000D_ Rare mutation in prolidase gene causes deficiency leading to massive imidodipeptiduria, elevated proline-containing dipeptides in plasma, recurrent infections, mental retardation and skin lesions._x000D_ Unit Definition_x000D_ One unit will hydrolyze 1.0 μmole of Gly-Pro per min at pH 8.0 at 40 °C._x000D_ Physical form_x000D_ Supplied as a lyophilized powder containing Tris buffer salt and MnCl2.