Description General description A potent and cell-permeable proteasome inhibitor. Inhibits the chymotrypsin-like activity of the pituitary multicatalytic proteinase complex (MPC; Ki = 460 nM). Does not inhibit the peptidylglutamyl-peptide hydrolyzing (PGPH) activity of MPC even at concentrations of 200 µM. Also blocks the decay of IκB-α and IκB-β proteins in exponentially growing WEHI 231 cells. A potent, cell-permeable, and reversible proteasome inhibitor. Inhibits the chymotrypsin-like activity of the pituitary multicatalytic proteinase complex (MPC; Ki = 460 nM). Does not inhibit the peptidyl-glutamyl peptide hydrolyzing activity of MPC even at concentrations of 200 µM. Also blocks the decay of IκB-α and IκB-β proteins in exponentially growing WEHI 231 cells. Packaging 1, 5 mg in Plastic ampoule Biochem/physiol Actions Cell permeable: yes Primary Target MPC Product does not compete with ATP. Reversible: yes Target Ki: 460 nM against the pituitary multicatalytic proteinase complex (MPC) Warning Toxicity: Standard Handling (A) Sequence Z-Leu-Leu-Phe-CHO Reconstitution Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months. Other Notes Schauer, S.L., et al. 1998. J. Immunol. 160, 4398. Orlowski, M., et al. 1997. Biochemistry 36, 13946. Orlowski, M., et al. 1993. Biochemistry 32, 1563.