Description_x000D_ General description_x000D_ Carboxy-terminal FLAG-BAP Fusion Protein is a 466 amino acid C-terminal FLAG fusion protein of E.coli bacterial alkaline phosphatase (BAP)._x000D_ Application_x000D_ Carboxy-terminal FLAG-BAP™ Fusion Protein has been used in the immunoprecipitation of the reporter protein in human embryonic kidney (HEK) cell lysate and as a FLAG-tagged control protein in solid-phase binding assay of spermatogenic immunoglobulin superfamily protein (SgIGSF)._x000D_ Biochem/physiol Actions_x000D_ The FLAG sequence comprises of the eight-amino acid sequence AspTyrLysAspAspAspAspLys and is hydrophilic. FLAG fusion proteins are expressed in bacterial, yeast and mammalian cells. FLAG epitope-tagged bacterial alkaline phosphatase is employed in immunoaffinity purification. Alkaline phosphatase based fusion protein have wide clinical applications in immunodetection, enzyme immunoassay and enzyme-linked immunosorbent assay._x000D_ Other Notes_x000D_ Control protein_x000D_ Preparation Note_x000D_ Dilute the ANTI-FLAG M2 antibody solution to 10 mg/ml_x000D_ Legal Information_x000D_ FLAG-BAP is a trademark of Sigma-Aldrich Co. LLC