Description_x000D_ General description_x000D_ This plasmid contains a multiple cloning site that is upstream of a minimal promoter that is upstream of the human secreted alkaline phosphatase (SEAP) reporter gene. This plasmid can be used to insert specific DNA sequences that will endow the minimal promoter with specific biochemical properties such as tissue specificity or transcription factor specific activation._x000D_ Promoter Expression Level:_x000D_ Application_x000D_ Multiple Cloning Site Notes: There is a start codon in the NcoI site in the MCS that can be removed by digestion with KpnI if required. The MCS for gene insertions extends from NotI to XbaI`But there are built in Shine-Dalgarno and Kozak ribosomal entry site sequences aligned with the start codon in the NcoI site. The ClaI and NheI sIt`s have other functions and allow the insertion of sequences from our other catalogue plasmids (such as IRES sequences and second promoters)._x000D_ The BsgI and BseRI restriction sIt`s in the plamsid cleave within the stop codon in the XbaI site in the MCS. This can allow retrospective fusion of coding sequences to our peptide tag range if your genes stop codon is positioned here._x000D_ Sequence_x000D_ Quick-reference Plasmid Map_x000D_ Please select the file type you require. For reference most cloning programs will import a .gb (Genbank) file and will show all of the plasmids features automatically when downloaded and imported._x000D_ Genebank Vector Sequence File_x000D_ FASTA Vector Sequence File_x000D_ Full Plasmid Map_x000D_ Analysis Note_x000D_ To view the Certificate of Analysis for this product, please visit www.oxfordgenetics.com._x000D_ Other Notes_x000D_ Looking for more vector options to move your experiments forward faster? Consider a custom cloning vector designed and built by Oxford Genetics™. Find out more at Oxford Genetics - Sigma's partner for cloning and expression vectors for molecular biology and synthetic biology applications._x000D_ Legal Information_x000D_ Oxford Genetics is a trademark of Oxford Genetics Ltd