Description_x000D_ General description_x000D_ This plasmid has been designed so that sequences can inserted between two reporter genes (renilla and firefly luciferase) to allow the relative transcription level in both directions to be determined by measuring the reporter luminescence. Dual luciferase reporter assay kits are available from other providers that are compatible with this plasmid. The plasmid only contains a Bgl2 site between the two genes to allow insertion._x000D_ Promoter Expression Level:_x000D_ Application_x000D_ This plasmid contains two luciferase reporter genes (Firefly and Renilla) in opposing directions with a Bgl2 restriction site in between the two genes to allow promoters to be inserted. This allows the transcriptional level of DNA sequences and promoters to be determined in both directions by inserting the sequences into the Bgl2 restriction site. If the sequence you would like to insert contains a Bgl2 site fragments with BamHI also have the same overhangs and can be ligated into this plasmid cut with Bgl2._x000D_ Sequence_x000D_ Quick-reference Plasmid Map_x000D_ Please select the file type you require. For reference most cloning programs will import a .gb (Genbank) file and will show all of the plasmids features automatically when downloaded and imported._x000D_ Genebank Vector Sequence File_x000D_ FASTA Vector Sequence File_x000D_ Full Plasmid Map_x000D_ Analysis Note_x000D_ To view the Certificate of Analysis for this product, please visit www.oxfordgenetics.com._x000D_ Other Notes_x000D_ Looking for more vector options to move your experiments forward faster? Consider a custom cloning vector designed and built by Oxford Genetics™. Find out more at Oxford Genetics - Sigma's partner for cloning and expression vectors for molecular biology and synthetic biology applications._x000D_ Legal Information_x000D_ Oxford Genetics is a trademark of Oxford Genetics Ltd