Description_x000D_ General description_x000D_ This vector is a standard Saccharomyces cerevisiae yeast expression plasmid with the exception that it has been modified to allow the direct cloning of gene fragments that have been designed to fit with any of our peptide tag plasmid range. This can be useful if you have created a peptide fusion but you would also like to create a non-tagged gene variant. The multiple cloning site contains two BsgI and BseRI sIt`s that produce overhangs that are compatible with gene designed using our SnapFusion primer designer (see the cloning tab for this product). The promoter in this plasmid (TEF1) is a strong constitutive yeast promoter._x000D_ Promoter Expression Level: This plasmid contains the yeast translation elongation factor 1 promoter. It is the strongest promoter that we provide for expression in Saccharomyces cerevisiae._x000D_ Sequence_x000D_ Quick-reference Plasmid Map_x000D_ Please select the file type you require. For reference most cloning programs will import a .gb (Genbank) file and will show all of the plasmids features automatically when downloaded and imported._x000D_ Genebank Vector Sequence File_x000D_ FASTA Vector Sequence File_x000D_ Full Plasmid Map_x000D_ Analysis Note_x000D_ To view the Certificate of Analysis for this product, please visit www.oxfordgenetics.com._x000D_ Other Notes_x000D_ Looking for more vector options to move your experiments forward faster? Consider a custom cloning vector designed and built by Oxford Genetics™. Find out more at Oxford Genetics - Sigma's partner for cloning and expression vectors for molecular biology and synthetic biology applications._x000D_ Legal Information_x000D_ Oxford Genetics is a trademark of Oxford Genetics Ltd