Description_x000D_ General description_x000D_ Sigma’s pUC18 DNA Hae III Digest contains 11 fragments from 11 - 587 bp. About three ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel._x000D_ Application_x000D_ pUC18 DNA Hae III Digest has been used as a DNA marker to compare the internal control developed for the competitive polymerase chain reaction (PCR) for the detection of Butyrivibrio fibrisolvens in the rumen._x000D_ Components_x000D_ Sigma’s pUC18 DNA Hae III Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA._x000D_ Other Notes_x000D_ Contains 11 fragments, 11–587 bp_x000D_ For optimal resolution, the recommended agarose gel concentration is 2.5%._x000D_ Preparation Note_x000D_ Plasmid pUC18 DNA was digested with Hae III to completion, then purified by phenol extraction.