Description_x000D_ Biochem/physiol Actions_x000D_ Puromycin aminonucleoside is an aminonucleoside derivative of the known antibiotic puromycin. Puromycin aminonucleoside has been used in nephrology research, studying focal and segmental glomerulosclerosis, and in the induction of nephrosis in rats. Rats with puromycin aminonucleoside-induced nephrotic syndrome were used to study the excretion of sodium and NOx metabolites._x000D_ Puromycin aminonucleoside has also been used to probe endothelial glycosaminoglycan synthesis in cultured glomerular endothelial cells and their relation to cell permeability. A puromycin aminonucleoside nephrosis model of rat glomerular disease was also used to study the role of the neuron-specific ubiquitin C-terminal hydrolase protein gene product 9.5 (PGP 9.5)._x000D_ Preparation Note_x000D_ Puromycin aminonucleoside solution is provided at 10 mg/mL in water and could be further diluted in aqueous buffers to a working concentration of 10 μg/mL (1:1000).