Description_x000D_ General description_x000D_ HepaRG is a human hepatoma cell line. The cells possess a pseudodiploid karyotype and have been characterized as an oval ductular bipotent hepatic cell line as they have the ability to differentiate into both biliary and hepatocyte lineages in the presence of DMSO. HepaRG PXR KO cells express the major xenobiotic sensors (CAR and AhR), drug transporters, phase I and II drug metabolizing enzymes as well as key hepatic transcription factors involved in stress response pathways._x000D_ Application_x000D_ See technical bulletin for detailed protocols_x000D_ Features and Benefits_x000D_ HepaRG cells are the most metabolically active human hepatocyte cell line developed to date. These cells are suitable for a wide variety of studies for drug metabolism, CYP induction, metabolism-mediated toxicity, transporter, and hepatotoxicity._x000D_ • Sigma′s HepaRG PXR Knockout (KO) allows conclusive identification of CYP3A4 induction through PXR activation._x000D_ Zinc finger nucleases (ZFN) mediated Knock-out of NR1I2 (PXR) gene._x000D_ • The frame-shift mutation of NR1I2 gene was confirmed by fragment length analysis and DNA sequencing._x000D_ • Loss of functionality was confirmed by loss of response to inducers of cytochrome P450 activity._x000D_ Quality_x000D_ Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, cytochrome oxidase I (COI) analysis for cell line species confirmation_x000D_ Legal Information_x000D_ These products are covered by the License Agreement as described in Exhibit 1 and 2, in the technical bulletin._x000D_ HepaRG is a trademark of BioPredic International company