Description_x000D_ Application_x000D_ Raf-1 is a serine/threonine protein kinase which is involved in the MAPK signal transduction pathway, a pathway that is involved in the development and progression of cancer. Raf-1 kinases are used to study potential cancer treatments as well as the responses to cancer treatments ._x000D_ Biochem/physiol Actions_x000D_ Raf-1 activates the protein kinases MEK1 and MEK2. MEK1 and MEK2 are phosphorylated to activate protein kinases ERK1 and ERK2. Activated ERKs play an important role in cell division, apoptosis, cell differentiation, and cell migration ._x000D_ Unit Definition_x000D_ One unit will activate MEK B substrate by 10% per min at pH 7.0 at 30 °C._x000D_ Physical form_x000D_ Solution in 50 mM Tris-HCl, pH 7.0 buffer containing 14 mM β-mercaptoethanol, 1 mM benzamidine, 0.1 mM PMSF, 1 mM EDTA, and 10% glycerol.