Description_x000D_ General description_x000D_ REDAccuTaq LA DNA polymerase allows for quick recognition in high throughput applications as well as direct loading of amplification products onto agarose gels for electrophoresis. The inert red dye has no effect on automated sequencing, restriction enzyme digestion, ligation, or other downstream manipulations. However, the PCR product is easily separated from the dye by standard purification methods._x000D_ Application_x000D_ Produce long DNA amplicons for:_x000D_ • Mutation analysis_x000D_ • Cloning genes_x000D_ • DNA sequencing_x000D_ • cDNA library generation_x000D_ Features and Benefits_x000D_ • Increased fidelity, up to 6.5× that of Taq DNA polymerase_x000D_ • Efficiently and accurately produce amplicons up to 22 kb on genomic templates and up to 40 kb on less complex templates such as lambda or bacterial DNA_x000D_ • Visual confirmation that enzyme has been added, and proper mixing has occurred_x000D_ • No additional loading dyes needed. A post-reaction aliquot can be directly used on an agarose gel for electrophoresis_x000D_ Packaging_x000D_ The enzyme is provided with an optimized 10× reaction buffer for enhanced amplification of complex templates._x000D_ Other Notes_x000D_ REDAccuTaq® LA DNA polymerase is a blend of Sigma′s high quality Taq DNA polymerase, a small amount of an additional polymerase that exhibits 3′→5′ exonuclease or proofreading activity, and our inert red dye. By blending the Taq with the right amount of this proofreading enzyme, misincorporation errors are corrected, producing PCR amplicons that are longer and more accurate._x000D_ Store REDAccuTaq LA DNA Polymerase and AccuTaq LA 10x Buffer at –20 °C._x000D_ Unit Definition_x000D_ One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min. at 74 °C._x000D_ Legal Information_x000D_ Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA._x000D_ REDAccuTaq is a registered trademark of Sigma-Aldrich Co. LLC