Description_x000D_ General description_x000D_ Renin, also known as angiotensinogenase, is an aspartyl protease that cleaves angiotensinogen to form angiotensin I, the initial and rate-limiting step of the renin-angiotensin system (RAS). This system plays a critical role in the regulation of blood pressure and fluid balance. Increased renin activity can result in hypertension due to increased vasoconstriction and fluid retention. Inhibitors of renin can be used as a treatment for hypertension._x000D_ Application_x000D_ Renin Assay Kit has been used to determine the activity of renin._x000D_ Suitability_x000D_ The Renin Assay Kit provides a convenient and simple method for screening renin activity in a variety of samples or for screening renin inhibitors._x000D_ Principle_x000D_ Renin activity is determined using a proprietary fluorescence resonance energy transfer (FRET) peptide containing a fluorescence quencher. Cleavage of the peptide by renin results in the production of a fluorescent product (λex = 540/λem = 590 nm). This assay is ~50-fold more sensitive than the EDANS/DABCYL-based assay.