Description_x000D_ Specificity_x000D_ Recognition sequence: 5′-C/C(A,T)(T,A)GG-3′_x000D_ Ligation and recutting results: After 2-10-fold Sty I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut._x000D_ Heat inactivation: Inactivated at 65 °C for 15 minutes._x000D_ Other Notes_x000D_ Supplied with 10x Restriction Endonuclease Buffer SH (B3657)._x000D_ Physical form_x000D_ Solution in 20 mM Tris-HCl, pH 7.5, 0.1 mM EDTA, 50 mM KCl, 10 mM 2-mercaptoethanol, 0.1% gelatine (v/v), 0.01% polydocanol (v/v), 50% glycerol (v/v) at 4 °C