Description_x000D_ General description_x000D_ Ribonucleases do not hydrolyze DNA, because the DNA lacks 2′-OH groups essential for the formation of cyclic intermediates. RNase can hydrolyze RNA from protein samples. Pancreatic RNase A specifically cleaves at the 3′-side of pyrimidine (uracil or cytosine) phosphate bonds._x000D_ Application_x000D_ Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. It is also used in RNA sequence analysis and protection assays._x000D_ Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. Ribonuclease A is used for RNase protection assays, to remove unspecifically bound RNA, analysis of RNA sequences, to hydrolyze RNA contained in protein samples, and the purification of DNA. Ribonuclease A from bovine pancreas has been used in a study to determine that both PDI and PDIp can attack the native disulfide bonds in thermally-unfolded RNase and form stable disulfide-linked complexes. Ribonuclease A from bovine pancreas has also been used in a study to investigate the stepwise deamidation of ribonuclease A at five sites._x000D_ Packaging_x000D_ 1 g in glass bottle_x000D_ 100, 250, 500 mg in glass bottle_x000D_ 10 mg in poly bottle_x000D_ Features and Benefits_x000D_ • RNase protection assays_x000D_ • Remove unspecifically bound RNA_x000D_ • Analysis of RNA sequences_x000D_ • Hydrolyze RNA contained in protein samples_x000D_ • Purification of DNA_x000D_ Preparation Note_x000D_ A further chromatographic purification of R5503 to yield essentially pure RNAse A_x000D_ Analysis Note_x000D_ Protein determined by E1%/280_x000D_ RNase A purity determined by SDS-PAGE