Description_x000D_ General description_x000D_ Ribonucleases do not hydrolyze DNA, because the DNA lacks 2′-OH groups essential for the formation of cyclic intermediates. RNase can hydrolyze RNA from protein samples. Pancreatic RNase A specifically cleaves at the 3′-side of pyrimidine (uracil or cytosine) phosphate bonds. The ribonuclease (RNase) gene is mapped to human chromosome 14._x000D_ Application_x000D_ Ribonuclease A from bovine pancreas has been used to treat human glioblastoma U251 cell line prior to harvesting, for flow cytometry analysis, T suppressor cells and embryonic stem cell chromatin._x000D_ Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. It is also used in RNA sequence analysis and protection assays._x000D_ Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. Ribonuclease A is used for RNase protection assays, to remove unspecifically bound RNA, analysis of RNA sequences, to hydrolyze RNA contained in protein samples, and the purification of DNA. Ribonuclease A from bovine pancreas has been used in a study to assess the mechanism of heavy metal ions on RNase activity. Ribonuclease A from bovine pancreas has also been used in a study to investigate the performance of oligomeric poly(diallyldimethylammonium chloride) as displacer for cation-exchange displacement chromatography of proteins._x000D_ Biochem/physiol Actions_x000D_ The structure of Ribonuclease A (RNase A) comprises of four disulfide and catalytic triad of two histidines crucial for its functionality. RNase A displays three-dimensional domain swapping of the α-helical N-terminal and the C-terminal β-strand. It can exist as dimer or oligomers. The level of RNase is elevated in cancers and infectious diseases. RNase A family of enzymes serve as potential drug targets. The homolog and variants of RNase A are potential chemotherapeutic agents._x000D_ Features and Benefits_x000D_ • RNase protection assays_x000D_ • Remove unspecifically bound RNA_x000D_ • Analysis of RNA sequences_x000D_ • Hydrolyze RNA contained in protein samples_x000D_ • Purification of DNA_x000D_ Physical form_x000D_ Solution in 0.2 M sodium phosphate buffer, pH 6.4_x000D_ Preparation Note_x000D_ A further chromatographic purification of R5503 to yield essentially pure RNAse A_x000D_ Analysis Note_x000D_ Protein determined by E1%/280_x000D_ RNase A purity determined by SDS-PAGE