Description_x000D_ General description_x000D_ Ribonuclease inhibitor works to inhibit RNase activity by forming a tight, non-covalent 1:1 complex. It is derived from E. coli that express portions of the human placental ribonuclease inhibitor. It inhibits RNases A, B, and C. It will not inhibit RNases H, 1, T1, S1 Nuclease, SP6, T7 RNA Polymerase, T3 RNA Polymerase, AMV Reverse Transcriptase, M-MLV Reverse Transcriptase, or Taq Polymerase. The inhibitor can be removed by phenol extraction or by heating to 65°C for 10 minutes._x000D_ Application_x000D_ Useful for in vitro inhibition of ribonucleases, including procedures like cDNA synthesis, RT-PCR, and in vitro transcription and translation._x000D_ Suitable for use in_x000D_ • in vitro inhibition of ribonucleases_x000D_ • cDNA synthesis_x000D_ • RT-PCR_x000D_ • in vitro transcription and translation_x000D_ Biochem/physiol Actions_x000D_ Inhibits RNase by forming a tight, non-covalent 1:1 complex._x000D_ Components_x000D_ Ribonuclease Inhibitor is provided as a solution in 20 mM HEPES-KOH( pH 7.6), 50 mM KCl, 8 mM DTT and 50% glycerol._x000D_ Unit Definition_x000D_ One unit will reduce the activity of 5 ng of ribonuclease A by 50% in a cytidine 2′:3′-cyclic monophosphate system._x000D_ Physical form_x000D_ Solution in 20 mM HEPES-KOH, pH 7.6, 50 mM KCl, 8 mM DTT and 50% glycerol.